rabbit anti ube2e3 Search Results


mouse monoclonal anti ube2e3  (Novus Biologicals)
93
Novus Biologicals mouse monoclonal anti ube2e3
Mouse Monoclonal Anti Ube2e3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti ube2e3/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
mouse monoclonal anti ube2e3 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
anti ube2e3  (Bioss)
91
Bioss anti ube2e3
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Anti Ube2e3, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ube2e3/product/Bioss
Average 91 stars, based on 1 article reviews
anti ube2e3 - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier
mouse anti ube2e3  (OriGene)
99
OriGene mouse anti ube2e3
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Mouse Anti Ube2e3, supplied by OriGene, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti ube2e3/product/OriGene
Average 99 stars, based on 1 article reviews
mouse anti ube2e3 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
mouse monoclonal anti ube2e3  (Proteintech)
93
Proteintech mouse monoclonal anti ube2e3
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Mouse Monoclonal Anti Ube2e3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti ube2e3/product/Proteintech
Average 93 stars, based on 1 article reviews
mouse monoclonal anti ube2e3 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
anti-ube2e3 antibody  (Cocalico Inc)
90
Cocalico Inc anti-ube2e3 antibody
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Anti Ube2e3 Antibody, supplied by Cocalico Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ube2e3 antibody/product/Cocalico Inc
Average 90 stars, based on 1 article reviews
anti-ube2e3 antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti-ube2e3 ( α -2e3)  (Becton Dickinson)
90
Becton Dickinson anti-ube2e3 ( α -2e3)
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Anti Ube2e3 ( α 2e3), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ube2e3 ( α -2e3)/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
anti-ube2e3 ( α -2e3) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
snrpd1 antibody  (Bio-Techne corporation)
93
Bio-Techne corporation snrpd1 antibody
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Snrpd1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/snrpd1 antibody/product/Bio-Techne corporation
Average 93 stars, based on 1 article reviews
snrpd1 antibody - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
mouse-anti-ube2e3  (Millipore)
90
Millipore mouse-anti-ube2e3
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Mouse Anti Ube2e3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse-anti-ube2e3/product/Millipore
Average 90 stars, based on 1 article reviews
mouse-anti-ube2e3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
peroxidase-conjugated anti-flag  (Millipore)
90
Millipore peroxidase-conjugated anti-flag
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Peroxidase Conjugated Anti Flag, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase-conjugated anti-flag/product/Millipore
Average 90 stars, based on 1 article reviews
peroxidase-conjugated anti-flag - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
peroxidase-conjugated anti-myc  (Millipore)
90
Millipore peroxidase-conjugated anti-myc
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Peroxidase Conjugated Anti Myc, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase-conjugated anti-myc/product/Millipore
Average 90 stars, based on 1 article reviews
peroxidase-conjugated anti-myc - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
mouse anti-polyubiquitin  (Biomol GmbH)
90
Biomol GmbH mouse anti-polyubiquitin
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Mouse Anti Polyubiquitin, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-polyubiquitin/product/Biomol GmbH
Average 90 stars, based on 1 article reviews
mouse anti-polyubiquitin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
rabbit anti lc3b  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc rabbit anti lc3b
(A) The expression of <t>UBE2E3</t> in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.
Rabbit Anti Lc3b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti lc3b/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
rabbit anti lc3b - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

Image Search Results


(A) The expression of UBE2E3 in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.

Journal: PeerJ

Article Title: UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging

doi: 10.7717/peerj.12253

Figure Lengend Snippet: (A) The expression of UBE2E3 in 7862 normal tissues of GTEx. (B) The correlation analysis of UBE2E3 with osteogenesis related genes in GTEx. (C) The correlation analysis of COL1A1 and UBE2E3 in GTEx. (D, E) Heatmap of single cell sequencing of rat BMSCs. (F) UBE2E3 expression of human BMSCs from GSE35956 and GSE35958 . (G) qRT-PCR analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (H) Western blot analyses of UBE2E3 levels in old mouse BMSCs and young mouse BMSCs. (I) qRT-PCR analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. (J) Western blot analyses of UBE2E3 levels in increasing numbers of passages of mice BMSCs. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.

Article Snippet: Next, membranes were blocked at room temperature with 5% defatted milk for 1 h. Then the membrane was incubated with anti- UBE2E3 (bs-8352R, 1:500; Bioss), anti- GAPDH (10494-1-AP,1:5000; Proteintech) overnight at 4 °C.

Techniques: Expressing, Sequencing, Quantitative RT-PCR, Western Blot, Standard Deviation

(A) Knockdown efficiency of UBE2E3 was verified by qRT-PCR. (B) Knockdown efficiency of UBE2E3 was verified by western blot. (C) β-Gal staining and quantification of senescent BMSCs in siUBE2E3 group and siNC group. (D) The relative mRNA expression of p16 and p21 between siUBE2E3 group and siNC group. (E) ALP and ARS staining results in siUBE2E3 group and siNC group, with the quantitative analysis. (F) The relative mRNA expression of RUN2, ALP, SP7 and BGLAP between siUBE2E3 group and siNC group. Error bars showed standard deviation. * P < 0.05, ** P < 0.01.

Journal: PeerJ

Article Title: UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging

doi: 10.7717/peerj.12253

Figure Lengend Snippet: (A) Knockdown efficiency of UBE2E3 was verified by qRT-PCR. (B) Knockdown efficiency of UBE2E3 was verified by western blot. (C) β-Gal staining and quantification of senescent BMSCs in siUBE2E3 group and siNC group. (D) The relative mRNA expression of p16 and p21 between siUBE2E3 group and siNC group. (E) ALP and ARS staining results in siUBE2E3 group and siNC group, with the quantitative analysis. (F) The relative mRNA expression of RUN2, ALP, SP7 and BGLAP between siUBE2E3 group and siNC group. Error bars showed standard deviation. * P < 0.05, ** P < 0.01.

Article Snippet: Next, membranes were blocked at room temperature with 5% defatted milk for 1 h. Then the membrane was incubated with anti- UBE2E3 (bs-8352R, 1:500; Bioss), anti- GAPDH (10494-1-AP,1:5000; Proteintech) overnight at 4 °C.

Techniques: Quantitative RT-PCR, Western Blot, Staining, Expressing, Standard Deviation

(A) Overexpression efficiency of UBE2E3 was verified by qRT-PCR. (B) Overexpression efficiency of UBE2E3 was verified by western blot. (C) β-Gal staining and quantification of senescent BMSCs in UBE2E3 overexpression group and the control group. (D) The relative mRNA expression of p16 and p21 between UBE2E3 overexpression group and the control group. (E) ALP and ARS staining results in UBE2E3 overexpression group and the control group, with the quantitative analysis. (F) The relative mRNA expression of RUN2, ALP, SP7 and BGLAP between UBE2E3 overexpression group and the control group. Error bars showed standard deviation. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: PeerJ

Article Title: UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging

doi: 10.7717/peerj.12253

Figure Lengend Snippet: (A) Overexpression efficiency of UBE2E3 was verified by qRT-PCR. (B) Overexpression efficiency of UBE2E3 was verified by western blot. (C) β-Gal staining and quantification of senescent BMSCs in UBE2E3 overexpression group and the control group. (D) The relative mRNA expression of p16 and p21 between UBE2E3 overexpression group and the control group. (E) ALP and ARS staining results in UBE2E3 overexpression group and the control group, with the quantitative analysis. (F) The relative mRNA expression of RUN2, ALP, SP7 and BGLAP between UBE2E3 overexpression group and the control group. Error bars showed standard deviation. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Next, membranes were blocked at room temperature with 5% defatted milk for 1 h. Then the membrane was incubated with anti- UBE2E3 (bs-8352R, 1:500; Bioss), anti- GAPDH (10494-1-AP,1:5000; Proteintech) overnight at 4 °C.

Techniques: Over Expression, Quantitative RT-PCR, Western Blot, Staining, Expressing, Standard Deviation

(A) The correlation of NQO1 and UBE2E3 in GSE35956 . (B) The correlation analysis of GCLC and UBE2E3 in GSE35956 . (C) The correlation analysis of GCLC and UBE2E3 in GSE35958 . (D) The correlation of GCLM and UBE2E3 in GSE35958 . (E) Representative images of BMSCs treated with siNC and siUBE2E3, and stained with DAPI and antibody against Nrf2. Scale bar, 50 µm. (F) NQO1, GCLC and GCLM expression between siUBE2E3 group and siNC group as analyzed by qRT-PCR. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.

Journal: PeerJ

Article Title: UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging

doi: 10.7717/peerj.12253

Figure Lengend Snippet: (A) The correlation of NQO1 and UBE2E3 in GSE35956 . (B) The correlation analysis of GCLC and UBE2E3 in GSE35956 . (C) The correlation analysis of GCLC and UBE2E3 in GSE35958 . (D) The correlation of GCLM and UBE2E3 in GSE35958 . (E) Representative images of BMSCs treated with siNC and siUBE2E3, and stained with DAPI and antibody against Nrf2. Scale bar, 50 µm. (F) NQO1, GCLC and GCLM expression between siUBE2E3 group and siNC group as analyzed by qRT-PCR. Error bars showed standard deviation. * P < 0.05, *** P < 0.001.

Article Snippet: Next, membranes were blocked at room temperature with 5% defatted milk for 1 h. Then the membrane was incubated with anti- UBE2E3 (bs-8352R, 1:500; Bioss), anti- GAPDH (10494-1-AP,1:5000; Proteintech) overnight at 4 °C.

Techniques: Staining, Expressing, Quantitative RT-PCR, Standard Deviation

High expression of UBE2E3 contributes to nuclear translation of Nrf2 and its high activity. With age, the expression of UBE2E3 decreases and nuclear accumulation of Nrf2 and its activity are restrained, leading to cell senescence and reduced osteogenic differentiation potential of BMSCs.

Journal: PeerJ

Article Title: UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging

doi: 10.7717/peerj.12253

Figure Lengend Snippet: High expression of UBE2E3 contributes to nuclear translation of Nrf2 and its high activity. With age, the expression of UBE2E3 decreases and nuclear accumulation of Nrf2 and its activity are restrained, leading to cell senescence and reduced osteogenic differentiation potential of BMSCs.

Article Snippet: Next, membranes were blocked at room temperature with 5% defatted milk for 1 h. Then the membrane was incubated with anti- UBE2E3 (bs-8352R, 1:500; Bioss), anti- GAPDH (10494-1-AP,1:5000; Proteintech) overnight at 4 °C.

Techniques: Expressing, Activity Assay